Methods: Forty-five patients were studied twice in the same week, i.e. once after the long and short interdialytic intervals, respectively. The four BI methods used were: (a) calf normalized resistivity (CNR) at a 5-kHz frequency, (b) whole-body multifrequency BI spectroscopy (MF-BIS) to estimate the normal hydration weight (NHWWBM), (c) whole-body MF-BIS to estimate the ratio of extracellular volume to total body water (wECV/wTBW), and (d) whole-body single-frequency (50 kHz) BI analysis to
compute the ratio of ECV (sfECV) to TBW (sfTBW). Results: The relationship (slope of the regressive line) between relative changes (%) in the above mentioned four BI parameters and differences in weight (kg) was most pronounced with CNR (5.2 +/- 1.6%/kg), followed by wECV/wTBW (1.7 +/- 0.7%/kg) and NHW WBM (0.73 +/- 0.2%/kg). Changes in sfECV/sfTBW and differences in weight were not correlated. Conclusions: KU-57788 cell line CNR is more sensitive than whole-body BIS for detecting differences in fluid status. (C) 2014 S. Karger AG, Basel”
“Purpose: To investigate lysates from Acacia nilotica pods for their antimicrobial and cytotoxic activities against a variety of extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae as well as methicillin resistant Staphylococci aureus (MRSA).
Methods: ESBLs-producing E. coli and Klebsiella spp were
isolated from clinical and environmental specimens and incubated with Acacia nilotica pod lysates. The bactericidal activity and cytotoxic effects of the lysates were evaluated while fast protein liquid chromatography (size exclusion FPLC)
MK2206 was used to identify the various compounds showing bactericidal activity.
Results: The lysates showed remarkable bactericidal properties, killing almost 100 % of the bacteria they were tested against, including neuropathogenic Escherichia coli, MRSA, and Klebsiella spp. MK-4827 The bactericidal activity was heat-resistant and showed minimal cytotoxic effects on human brain microvascular endothelial cells. FPLC revealed eight peaks, with three of them representing compounds that had maximum bactericidal activity against all the tested isolates, but showed < 30 % host cell cytotoxicity.
Conclusion: The lysate of Acacia nilotica pods is a potentially good candidate for the therapy of antibacterial-resistant bacteria, and would therefore require further studies.”
“The antioxidant activities, total phenolic contents and chemical compositions of extracts from four Cameroonian woods were investigated. The antioxidant activities were evaluated using the scavenging of 2-2-diphenyl-1-picrylhydrazyl radical (DPPH.) and the oxygen uptake method. The total phenolic content (TPC) was evaluated according to the Folin-Ciocalteu assay in which gallic acid was used as standard. Condensed tannins were evaluated by using the Stiasny method. Chemical compositions of the extracts were evaluated by GC-MS.