05) (Fig 4D), and CBDL rats (maximum at 5 × 10−5M: 142 ± 14 ve

05) (Fig. 4D), and CBDL rats (maximum at 5 × 10−5M: 14.2 ± 1.4 versus 9.7 ± 1.1 mm Hg in

HC; P = 0.04) (Fig. 4B). 4 CCl4 and CBDL cirrhotic livers exhibited an increase of oxidative stress compared with control rats (Fig. 5A). CIH was associated with a higher degree of oxidative stress in the liver of control (Fig. 5B), CCl4 cirrhotic rats (Fig. 5C) and CBDL rats (Fig. 5D), as indicated by increased content of 3-NT. In control rats these results were associated with a significantly increase in eNOS phosphorylation (Fig. 6A), whereas cirrhotic rats exhibited similar eNOS and p-eNOS (Fig. 6B, 6C). As expected, hepatic guanosine 3′,5′-cyclic monophosphate levels were significantly higher in HC control rats compared with HC cirrhotic rats (1.60 ± 0.92 versus 0.92 ± 0.14 pmol/mL, P < 0.05). However, there were no statistical differences between HC and CIH exposure in control rats (1.60 ± 0.23 versus 1.37 Fer-1 manufacturer ± 0.26 pmol/mL, P = 0.5) under the experimental conditions tested, although a trend was observed in CCl4 cirrhotic rats (0.92 ± 0.14 versus 0.60 ± 0.2 pmol/mL, P = 0.2). These data suggest that CIH exposure

induces oxidative stress and probably eNOS phosphorylation, which is impaired in cirrhotic rats provoking attenuation in vasodilation and hyperresponse to the vasoconstrictor Mtx. We used a conventional MK-2206 concentration rat model of CIH to study whether this well-known independent factor for systemic endothelial dysfunction could also provoke intrahepatic endothelial impairment in two different animal models of cirrhosis. Our study provided evidence that a short period of CIH exposure can influence the regulation of hepatic vascular tone in cirrhosis, exacerbating intrahepatic endothelial dysfunction.

In addition, our results suggest that CIH effects are more relevant as portal hypertension develops. The most relevant finding in our study is that the intrahepatic vasodilation response 上海皓元医药股份有限公司 to ACh was significantly attenuated in CCl4 cirrhotic rats exposed to CIH compared with HC rats. This result is in keeping with previous experiments showing that CIH exposure exerts similar hemodynamic effects in other vessels.5 In addition, a hyperresponse to Mtx was also observed in CCl4 and CBDL cirrhotic rats, which is also in agreement with previous data.6 An increase in oxidative stress was also found after CIH. Hence, theoretically the net result of increased oxidative stress and decreased eNOS activity accounts for the significant alteration in the vascular reactivity of cirrhotic livers. CIH (and OSAS), in addition to causing systemic hypertension,18 ischemic heart disease,19 and stroke,20 has organ-specific effects in the lung leading to pulmonary hypertension.21 In all these disorders, endothelial dysfunction has been identified as an early event.

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