3) The 5-HT4 agonist mosapride decreased the length and frequenc

3). The 5-HT4 agonist mosapride decreased the length and frequency of LDCs but markedly promoted distal colon propulsive activity through increasing RPMCs.4). 5-HT at low concentrations (∼ 5 uM) strongly inhibited all activities, likely due to direct action on muscle. 5). When segmentation occurs, it replaces RPMCs, it is slow at 3.6 short-lasting contractions/min and occurs in the mid and distal colon. Conclusion: LDCs are dependent on 5-HT3 receptor activation. 5-HT3 antagonists mostly reduce RPMCs and segmentations but RMPCs and segmentation do not require 5-HT3 receptor activation Crizotinib research buy and the motor patterns can increase in the presence of 5-HT3 antagonists. 5-HT4

receptor activation, promotes propulsion by creating short-lasting proximal LDCs and vigorous distal RPMCs. Key Word(s): 1. Colonic motility; 2. 5-HT4 receptor; 3. 5-HT3 receptor; 4. Motor patterns; Presenting Author: QIAN ZHANG Additional

Authors: JI-HONG CHEN, HE-SHENG LUO Corresponding Author: JI-HONG CHEN Affiliations: Department of Gastroenterology and Hepatology, Renmin Hospital of Wuhan University Objective: To explore Sorafenib ic50 the motor patterns and their features of distal colon in rats in vitro and provide evidences for human colonic motility and its mechanism. Methods: Combined the technics of organ bath, water-perfused manometric system and spatiotemporal Hydroxychloroquine purchase mapping with pharmaceutical intervention as well as fluid infusion, the motor activities of the distal colon in vitro and their neurogenic and myogenic features were investigated in 35 healthy Sprague Dawley rats. Results: Motor patterns like rhythmic propulsive motor

complexes, ripples, segmentation and long distance contractions (LDCs) were observed in the distal colon of healthy rats; LDCs could be spontaneous or induced by fluid infusion, and those which reached the distal colon formed various combinations with other motor patterns. Non-selective nerve blockers, tetrodotoxin and lidocaine, inhibited both the spontaneous and the fluid-infusion induced LDCs, changed the frequency and the propagation distance of motor complexes, promoted ripples and induced segmentation in the distal colon. In the presence of tetrodotoxin/lidocaine and bethanechol, long-term LDC-like motor patterns and retrograde contractions which generated from the anal end of the colon appeared. L-NNA inhibited the spontaneous and induced LDCs, also changed the patterns of motor complexes. Conclusion: Distal colon has various motor patterns in rats in vitro: LDCs with myogenic and neurogenic features; myogenic patterns as rhythmic propulsive motor complexes, ripples, segmentation and retrograde contractions. Key Word(s): 1. Distal Colon; 2. Motor Patterns; 3.

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