Protein sensitized T AL All cells to the t Dliche effects of glucocorticoids Of. In addition, we have tried to reveal the mechanism, tr by NTAL Gt induces cell death by prednisone. Functional T-cell receptor signaling COX Inhibitors is a hallmark of T cell ALL. NTAL is non-enzymatic signal molecule that is associated with the TCR. Therefore, initially we have Highest to TCR-induced signal transduction concentrated. After TCR stimulation, the cells expressing the protein NTAL show a Ph Genotype more energy and a gr Ere expression of the CD69 molecule on the cell Surface of cells NTAL negatively. In addition, increased Ht NTAL TCR-induced cell death. We have then examined the intracellular Ren signaling pathways. Proteins ERK, JNK and p38 MAPK are important signaling molecules in lymphocytes.
The basal phosphorylation of ERK, JNK and p38 MAPK is not between Jurkat / wt and different Jurkat / T cells NTAL. After TCR stimulation, we observed only minor Changes in the phosphorylation of JNK and no Ver Change of p38 MAPK, w While ERK is phosphorylated and strongly t t. In addition, show Jurkat / Alternative drivers NtAlt cells ERK phosphorylation significantly h Ago after TCR stimulation of Jurkat / wt cells. We conclude that increased Hte signaling through NTAL improved activation of ERK. We can k The hypothesis that is localized in the T-cell ALL, in NTAL Lipidfl S, binds to GRB2 molecules, and increased Ht the activation of the Ras / Raf / MEK / ERK signaling cascade. In an n Next step, we focused on the molecule ERK and R In cell death.
After TCR cross-linking, inhibition of ERK phosphorylation by the inhibitor U0126 blocked early cell death. When U0126 sp Ter applied, the kinetics of cell death Similar in cells treated with the fight against CD3 alone. We conclude S the fact that the events leading to cell death within the first 30 minutes after TCRadded cultures of Jurkat cells / weight and Jurkat / T cells measured 30 minutes before the start NTAL methylprednisolone, 48 hours, and cell death by flow cytometry . Unlike the original experiment without the intervention of ERK phosphorylation after inhibition of ERK by U0126, the percentage of surviving cells Like in Jurkat / wt and Jurkat / Alternative drivers NtAlt. Our experiments suggest that the mechanism leads to an increased Hten cell sensitivity to methylprednisolone treatment alternative driver h Here NtAlt activation of ERK kinase.
Discussion The exact mechanism for the regulation of sensitivity of t, or resistance to glucocorticoids Of is still not completely Ndig understood. In our previous study, we describe for the first time the correlation between strong expression of the adapter protein NTAL and better response to prednisone in the T-cell ALL. In this study best CONFIRMS, we showed that in vitro data and that the presence of the protein NTAL All sensitized T cells to the t Dliche effect of glucocorticoid Of. In addition, we have tried to reveal the mechanism, tr by NTAL Gt induces cell death by prednisone. Functional T-cell receptor signaling is a hallmark of T cell ALL. NTAL is non-enzymatic signal molecule that is associated with the TCR. Therefore, initially we have Highest to TCR-induced signal transduction concentrated. After TCR-stimulation, which show the protein expressing cells NTAL a Ph Genotype more energy and a gr Ere expression of the CD69 molecule on the surface Surface of the cell, the cell negative NTAL
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