CYT997 of treatment the number of cells in the G2 / M phase doubled

The cells were in the G1 phase and this is not changed substantially after 4 h treatment 4 methylcatechol GE. However, CYT997 by 16 clock only about 50% of the cells in the G1 phase, which is not within 24 hours of GE Was changed. There was no significant improve Change in the percentage of replication of cells after treatment 4 methylcatechol. About 10% of cells were in G2 / M at 0h time and it does not change after 4 h of treatment. However, after 16 h of treatment the number of cells in the G2 / M phase doubled, and 24 hours was about 35% of the cells in the G2 / M phase, in comparison to about 10% for 0h time. These results suggest that after 4 methylcatechol treatment, even when moving a big e number of cells from the G1 to S phase, they are in the G2 / M cell cycle block.
4 methylcatechol induces apoptosis in melanoma cells, metastatic cells, we followed the 4-methylcatechol by morphological observation Ver Changes discussed. We found that increasing doses of 4 methylcatechol characteristics of apoptosis, Including Show Lich budding from the cell membrane, condensed nuclear material followed by progressive cell rounding and lifting of the culture plate. Photographs of cells with 10 g / ml of 4 methylcatechol at 0h, 4h, 16h and 24h treated in Figure 5 show that the number of dead cells increased with time Ht. Subsequently End we have to determine annexin V-FITC test whether cell death was mediated by 4 methylcatechol apoptosis in these cells. In this essay bind necrotic cells and Annexin V-FITC. Propidium iodide was used to distinguish between lebensf HIGEN, early apoptotic and necrotic or late T to distinguish apoptotic cells.
Necrotic cells bound annexin V FITC and propidium iodide with Customised Rbt, w While propidium iodide by lebensf HIGEN and early apoptotic cells were excluded. The data were plotted for the early apoptotic cells and are shown here. The results of dose-response experiments show a dose- Independent increase in apoptosis in cells with increasing doses of 4 methylcatechol treated for 12 h. We found that the treatment was with 10 g subjected to / ml of 4 methylcatechol about 10% of the cells apoptosis and 20g/ml 4 methylcatechol there is a 2.5 fold increase in apoptotic cells as compared to 10 g / ml treatment. We have also conducted experiments on time course with 10 g / ml of 4 methylcatechol is shown in Figure 5.
The number of apoptotic cells at 2 minimum, based on about 10% after 6 and 24 hours was about 25% of cells positive for FITC annexin early apoptosis. Approximately 5-7% of the cells treated with 4 methylcatechol underwent necrosis. Taken together, these results indicate that treatment with 4 methylcatechol cell death by apoptosis in metastatic melanoma cell culture. 4 methylcatechol d mpft Survive apoptotic and anti-Western blotting was used to Ver Changes in the key pro and anti-apoptotic proteins and to determine survival. Either whole cell or mitochondrial extracts were prepared from cells that were treated with increasing concentration of 4 methylcatechol. In Figure 6, the H Height of the antiapoptotic protein Bcl-2 may need during the treatment with 4 methylcatechol from, w While the H He obtained the pro apoptotic Bax Shown ht. We have also found that the reduced amount of cytochrome c in mitochondrial extracts after treatment with 25