Figure 5 A typical FL micrograph of the as-deposited MS-C 20 binary LB film of ten layers. Red fluorescent image with 540-nm excitation (a); the schematic layered structure (b). Figure 6 shows the BF Kinase Inhibitor Library purchase microscopy image (a) and the FL microscopy image (red fluorescent image with 540-nm excitation) of the MS-C20 mixed LB film of ten layers after HTT (80°C, 60 min) (b) together with the schematic layered structure (c). Round-shaped domains are observed both by BF microscopy and FL microscopy and the domain sizes are reaching 100 μm in diameter. In our previous works, due
to insufficient color sensitivity and the resolution limit of the BF microscope, microstructures of the domains were not characterized sufficiently [18, ZIETDFMK 20–25]. However, from Figure 6a in the present work, it has been found that the bluish areas tend to be observed in round-shaped domains compared to areas outside. Furthermore, the bluish areas observed by BF microscopy (Figure 6a) are found to emit intense fluorescence compared to colorless areas, as shown in Figure 6a,b.
These results strongly indicate that the bluish areas emitting intense red fluorescence correspond to the crystallites of reorganized J-aggregates. Figure 6 A BF microscopy image and the FL microscopy image of the mixed MS-C 20 LB film. A BF microscopy image (a) and the FL microscopy image (red fluorescent image with 540-nm excitation) of the corresponding area (b) of the mixed MS-C20 LB film of ten layers after HTT (80°C, 60 min) with the schematic layered structure (c). It should be also CP690550 noted that there are two different types of domains observed
in Figure 6a,b. One type is of domains with rims of deeper blue (blue-rimmed domains), and the other type is of domains with rims Sinomenine of lighter blue (white-rimmed domains). As shown in Figure 6b, the fluorescence image shows that the emission from blue rims is more intense compared to areas inside, and on the other hand, the emission from white rims is less intense compared to areas inside. Diameters of blue-rimmed domains are reaching 100 μm or even greater, as seen in Figure 6a,b. On the other hand, diameters of white-rimmed domains are typically in the range of 40 to 60 μm, which are significantly small compared to blue-rimmed domains. In our previous works, we categorized the two types of domains as ‘dark-rimmed domains’ and ‘bright-rimmed domains’ [18, 22], which are now categorized as blue-rimmed domains and white-rimmed domains, respectively. Observations by BF microscopy and FL microscopy have revealed that the crystallites of J-aggregates exist in domains of both types in the mixed MS-C20 LB films after HTT. Furthermore, in blue-rimmed domains, the density of reorganized J-aggregate crystallites appears to be higher near domain boundaries compared to other areas.