In the water, the decreased gravitational force and increased cen

In the water, the decreased gravitational force and increased central venous pressure facilitate venous return, which in turn stretches the atrial chambers, increasing the expression and secretion of ANP [42]. This mechanism has also been demonstrated using SHR as an animal Dabrafenib price model [36]. However, in our study, the higher level of ANP in the plasma of the swimming trained group could be accounted for by either higher secretion or lower degradation. Because no alterations were observed in the storage of ANP or mRNA in the right and left atria of swimming trained rats, the higher plasma levels of ANP may be due to a decrease in degradation by NPRC in the kidneys and adipose

tissue. Our study found no change in the

plasma levels of PI3K Inhibitor Library ANP in the RN compared to the SD group. In contrast, previous data from running trained normotensive rats found changes in the plasma concentrations of ANP with no difference in the atria [16]. Differences in the intensity, the duration of training and the species of rat that was studied could be responsible for the differences that were found. Furthermore, another study of normotensive rats found that increases in the intensity of exercise were accompanied by increased plasma levels and concentrations of ANP in cardiomyocytes [29]. Besides showing the same methodological differences as in the previous study, the technique of cardiac ANP analysis and the time of collection (immediately vs. 48 h after the last session) may explain the differences found in our study. The alterations of plasma ANP levels cannot be attributed only to the atria’s ability to express, synthesize and secrete Flucloronide ANP. The plasma ANP levels may also be affected by its clearance by the NPR-C receptor.

Upon analyzing the expression of NPR-C in the kidneys, a significant decrease was found only in the SW group when compared to the SD group, which could explain the lower degradation of ANP and the increase in the plasma levels. However, there was a statistically significant decrease in NPR-A expression in the SW compared to the SD group. The downregulation of its receptor in the kidney could be the result of the increase in the ANP plasma levels. Shanshan et al. found an increased expression of NPR-A in the kidney of normotensive racing rats that were trained over eight weeks, although they found a decrease in NPR-C concentrations [38]. Moreover, Suda et al. did not find changes in the density of receptors for ANP in the kidneys, adrenal glands and lungs in Wistar normotensive rats that were trained on running for 6–7 weeks [44]. However, unlike ours, the Suda et al. study did not specifically evaluate each subtype of receptor for ANP; it assessed only the total density of the receptors.

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