Interestingly, our study validates the findings of a previous study which identified pyruvate dehydrogenase E1 α chain and
the protein encoded by MG_328 as phosphoproteins of M. genitalium. Recently, both these proteins were found on the surface of M. genitalium, thus suggesting the possibility that they can play a role in Y-27632 order M. genitalium-host interaction. What is intriguing in this study, however, is the reduced phosphorylation of a protein (PDH) in a phosphatase (MG208) deficient mutant of M. genitalium. Theoretically, if proteins are dephosphorylated by a specific phosphatase, then the expectation, in the absence of the phosphatase, is no change in the phosphorylation levels or increased levels of phosphorylation of proteins. In fact, the proteins identified in PrpC mutant of M. pneumoniae behave in this manner. The differentially phosphorylated
proteins identified in this mutant included RopE, an adhesion related protein P41, HMW3, MPN256 and MPN474. These proteins showed phosphorylation only in the PrpC mutant but not in the wild type. The contrasting situation in MG_207 tends to speculate that additional protein(s) with possible kinase property is affected in this mutant and this putative protein fails to phosphorylate some proteins in the mutant. However, this is only a hypothesis and requires additional studies for confirmation. Nevertheless, differential phosphorylation
www.selleckchem.com/products/ml323.html of proteins has been shown in the STP mutant strains of L. monocytogenes and S. pneumoniae. In addition to changes in protein phosphorylation, an STP (stp1) mutant of Group B Streptococcus has been shown stiripentol to have altered expression of 294 genes which included stk1, the gene encoding a major kinase . Table 1 Phosphorylated proteins identified by Mass spectrometry Spot numbera Protein name Gene symbol Gene Putative function 1 Pyruvate dehydrogenase E1 subunit α pdhA MG_274 Metabolism/surface protein 2 Pyruvate dehydrogenase E1 subunit α pdhA MG_274 Metabolism/surface protein 3 Putative cytoskeletal protein – MG_328 Cytoskeletal involvement 4 Conserved hypothetical protein – MG_281 Unknown 5 Thymidine phosphorylase deoA MG_051 Metabolism a Refers protein spots marked in Figure 3A and C. Ability of TIM207 strain to adhere and invade eukaryotic cells Since TIM207 strain showed differential phosphorylation of proteins, we speculated that this would have some impact on the adherence of this strain with eukaryotic cells. Consistent with this notion, we noticed that TIM207 strain adhered only partially to culture flasks when grown in SP-4 medium. The non-adherent mycoplasmas were seen as floating cell suspensions and this phenotype was highly reproducible.