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manuscript. All authors have read and approved the final manuscript.”
“Background The genus Legionella includes approximately 53 species [1], with Legionella pneumophila being the most common human pathogenic species and causing 90% of all outbreaks of Legionnaires’ disease (LD) in Europe [2]. Legionella species are ubiquitous microorganisms, occurring predominantly in aquatic environments, freshwaters and hot water systems [2], soils, potting soils [3], and composts [4]. Cooling towers, whirlpool spas and shower faucets could be the sources of contaminated bioaerosols, the inhalation of which is generally considered to cause LD outbreaks [2]. A variety of culture methods to detect Legionella species are used to analyze environmental samples [5].