Table S1. Mutated oligonucleotides used for the amplification of the point-mutated genes of the vanillate MT I of Acetobacterium dehalogenans. Table S2. Mutated oligonucleotides used for the amplification of the point-mutated genes of the veratrol MT I of Acetobacterium dehalogenans. Please note: Wiley-Blackwell is not responsible
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“The genome of Dictyostelium contains two novel hybrid-type polyketide synthases (PKSs) known as ‘Steely’; the Steely enzyme is formed by the fusion of type I and type III PKSs. One of these enzymes, SteelyB, is known to be responsible for the production of the stalk cell-inducing factor DIF-1 in vivo. On the other hand, the product(s) and expression pattern of SteelyA are not high throughput screening assay clearly understood, because there are two different reports associated with the in vitro products of SteelyA and its expression pattern. To solve this problem, we first examined the expression pattern using two different primer sets and found that it was quite similar to that shown in the dictyExpress database. stlA expression peaked at approximately 3 h and
declined, but showed a small peak around the end of development. Epigenetics inhibitor Next, we examined the in vivo product of SteelyA using a stlA null mutant and found that the mutant lacked 4-methyl-5-pentylbenzene-1,3-diol (MPBD). This null mutant showed aberrant, glassy sori, and most of the cells in the sori remained amoeba-like without a cell wall. This defect was restored by adding 200 nM of MPBD to the agar. These results indicate that SteelyA science produces MPBD in vivo and induces spore
maturation. Dictyostelium is an excellent model organism to study a developmental system that is regulated by the secreted signal molecules. Starvation triggers Dictyostelium cells to aggregate and form a multicellular mound, eventually forming a fruiting body that contains two types of differentiated cells: stalk and spore cells (Kessin, 2001). In the mound stage, cells begin to differentiate into prespore and prestalk cells at random positions. The differentiated cells sort out and form an anterior prestalk and posterior prespore zones at the slug stage (Kay & Thompson, 2009). The prestalk cell population is composed of several subtypes of cells (Williams, 1997), whereas the prespore cells are believed to be rather homogeneous. Under the appropriate conditions, the slug transforms into the final morphology, the fruiting body, which consists of a spore mass atop vacuolated and dead stalk cells. In Dictyostelium, the developmental process is a stress response triggered by starvation and the cell type differentiation process is mainly controlled by extracellular signals.