The phoCDET operon, codes for a high affinity phosphate transport

The phoCDET operon, codes for a high affinity phosphate transport system [44]. A phoB dependent control of phoCDET could be observed in S. meliloti [15] and in E. coli it could be shown that phoB is involved in the acid shock response [45]. Cluster F is almost exclusively composed of genes playing a role in chemotaxis and motility Cluster F consists of genes whose expression was continuously lowered during the time course experiment (Fig. 2F). It mainly comprises genes (10 of 22) belonging to chemotaxis and flagellar biosynthesis (flgB,

flgG, flgL, flgF, flgC, flgE, fliE, flbT, motA, mcpU). find more This phenomenon will be discussed in more detail later. Another gene in cluster F was lppB coding for a lipoprotein, which is a major outer membrane component was grouped in cluster F. A similar expression profile as those of the flagellar biosynthesis and chemotaxis genes confirms a possible co-regulation as was observed

in Salmonella enterica [46]. Cluster G consists of several genes involved in nitrogen uptake and utilization Cluster G consists of genes whose expression was transiently lowered between 8 and 18 minutes following Akt inhibitor the pH shift and afterwards returned nearly to the ground state (Fig. 2G). The genes nirB, nirD and narB were distributed to this cluster and are coding for nitrite and nitrate reductases forming ammonia from nitrate. A homologue of narB was found to be regulated by the low pH and microaerobiosis regulator ActR in S. medicae. A gene coding for an element of a nitrate import system (nrtB) could also be found in this cluster, while the remaining two elements of this system encoded by nrtA and nrtC were not included

in the analysis because their expression values were below the threshold for filtering. Additionally, genes coding for an ABC 5-Fluoracil research buy transport system (smb21707, smb20602, smb20603, smb20604 and smb20605) sharing homologies with amino acid and urea/short chain amide transport systems are present in cluster G. In addition to this transport system genes (smb20141, Evofosfamide chemical structure smb20142) of an ABC transport system homologous to the Dpp system from E. coli were also grouped in this cluster. This system is known for the import of dipeptides to provide the cell with essential amino acids, nitrogen and energy [47]. Cluster H is formed by genes with distinct biological functions and a high variation in their expression levels Cluster H consists of 13 genes that were transiently lower expressed on the very beginning of the time course (Fig. 2H). The proposed encoded functions of the genes in this cluster were found to be very diverse. A secreted peroxidase gene (sma1944) [48], a flagellar biosynthesis gene (fliP), a chemotaxis sensory gene (mcpW), a nodulation gene (nodP1) and several hypothetical protein encoding genes were identified to be in cluster H.

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