tuberculosis invasion. The confirmation of Rv0679c’s location in mycobacterial surface, together with the identification of a binding region formed by HABPs 30985-30987, suggest that this protein may be related to adhesion and/or invasion processes. In addition, such surface localization could be facilitating contact between the bacilli and its host cell, thereby leading to triggering the host’s immune response via interaction with host cell surface receptors . Conclusions The complexity of Mycobacterium tuberculosis as a pathogen and the variety of mechanisms that it uses for invading host cells
makes it necessary to develop an effective strategy to block the invasion of target cells. Our proposal is based on searching for fragments of different AUY-922 nmr proteins involved in the mycobacteria-host cell interaction. In our experience, sequences that bind specifically to target cells and that are capable of blocking invasion could be used as template to design peptides with ability to immunomodulate Selleckchem Midostaurin the protective response against tuberculosis. The immune response triggered against mycobacterial high-specific binding sequences could prevent invasion of target cells, either during a first encounter with the bacillum or during the reactivation of a latent infection. It has been reported that a considerable number of secreted proteins are
protective antigens and therefore have been considered as attractive candidates to develop subunit vaccines [43–46]. Moreover, they are hypothesized to mediate mycobacterial entry into the host cell . Traditionally, vaccine development has been founded on the humoral immune response, which involves antibody production and is mainly targeted against extracellular microorganisms, whereas the immune response against intracellular microorganisms is mainly driven by cellular immune mechanisms. In addition, the distinction between the Th1 and Th2 cellular immune responses is complex for some of the antigens or immunogens included in vaccines that induce cellular as well as humoral immune responses, and it is not yet clear the degree of independence
between antibody-mediated many and cell-mediated immune responses under physiological conditions [48, 49]. Considering the variety of broad interactions of B lymphocytes with cellular immunity, B cells could have a significant impact on the outcome of airborne challenge with M. tuberculosis as well as the resultant inflammatory response . Therefore, we expect for peptides of Rv0679c to induce an immune response where humoral and cellular immunity are not mutually excluded. The identification of Rv0679c HABPs capable of inhibiting target cell invasion by M. tuberculosis via host-cell receptor interactions supports their inclusion in further immunological studies in animal models aimed at evaluating their potential as components of a subunit-based antituberculous vaccine.