Mix 1: AKG (0 2 g·kg-1·d-1), prepared

with Na-AKG 144 66 

Mix 1: AKG (0.2 g·kg-1·d-1), prepared

with Na-AKG 144.66 mg·kg-1·d-1 (correspondingly 127.60 AKG mg·kg-1·d-1) and Ca-AKG 91.33 mg·kg-1·d-1 (correspondingly 72.40 mg·kg-1·d-1 AKG). Mix 2: BCKA (0.2 g·kg-1·d-1), composed of three components (α-ketoisocaproate, KIC, 47.4%; α-ketoisovalerate, KIV, 30.0% and α-ketomethylvalerate, KMV, 22.6%), check details prepared as follows: Na-KIC: 111.47 mg·kg-1·d-1 (correspondingly KIC 94.80 mg·kg-1·d-1), Ca-KIV: 69.73 mg·kg-1·d-1 (correspondingly KIV 60.00 mg·kg-1·d-1), Ca-KMV: 52.40 mg·kg-1·d-1 (correspondingly 45.20 mg·kg-1·d-1). Mix 3: Placebo of equivalent energy and sodium, as well as calcium salts of the same appearance as AKG and BCKA, composed of 235 mg·kg-1·d-1 glucose, 41.09 mg·kg-1·d-1 CaCO3, 38.02 mg·kg-1·d-1 NaHCO3. Determination GSI-IX manufacturer of the study parameters Observations were made at three points (Figure 1): before the training

as the baseline (Test 1), after the four weeks of training (Test 2) and at the end of one week of recovery (Test 3). The following parameters were determined. The weekly training time was calculated for both endurance DNA Damage inhibitor running and sprint running, according to the training protocol (Figure and Table 2). Table 2 Training data (mean ± SD)     Group     Control a-KG BCKA Training time (min/w)       Endurance training week 1 144 ± 12 143 ± 13 146 ± 14 week 2 130 ± 25 127 ± 33 140 ± 15 week 3 112 ± 48* 147 ± 10 127 ± 47 week 4 74 ± 54** 137 ± 30†† 122 ± 27†† sprint running week 1 44 ± 6 42 ± 4 42 3-oxoacyl-(acyl-carrier-protein) reductase ± 6 week 2 35 ± 8 37 ± 12 40 ± 6 week 3 30 ± 17 41 ± 5 34 ± 15 week 4 19 ± 17** 39 ± 12†† 35 ± 8† VO2max (ml·min-1·kg-1) before training 45.6 ± 7.3 47.1 ± 6.9 45.4 ± 5.1 after training 52.3 ± 6.2‡‡ 52.1 ± 7.2‡‡ 51.3 ± 5.2‡‡ after recovery 51.9 ± 8.3‡‡ 52.6 ± 7.1‡‡ 51.1 ± 5.1‡‡ Pmax (Watts) before training 365 ± 63 380 ± 59 369 ± 34 after training 377 ± 61 381 ± 56 374 ± 46 after recovery 381 ± 67 412 ± 49‡ 390 ± 29‡ PIAT (km/h) before training 9.6 ± 1.7 9.8 ± 2.2 9.9 ± 1.5 after training 10.8 ± 1.7‡ 10.6 ± 1.7‡ 10.6 ± 1.6‡ after recovery 10.5 ± 1.7 10.2 ± 2.1 10.4

± 1.4 AKG: α-keto glutarate; BCKA: branched-chain keto acids; min/w: training time in minutes each week; VO 2max : the maximum oxygen uptake measured on the cycle-ergometry; P max : the maximum power output on the cycle-ergometry; P IAT : the performance at the individual lactate threshold determined by treadmill test; T max_ISM : the maximum muscle torque by isometric measurement; P max_ISK : the maximum muscle performance by isokinetic measurement. * P<0.05 compared with that of 1st week; ** P<0.01 compared with that of 1st week; † P<0.05 compared with that of the control group; †† P<0.01 compared with that of the control group; ‡ P<0.05 compared with that before training; ‡‡ P<0.01 compared with that before training.

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