01), and the expression of SIGIRR had no significant difference between groups of Tim-1 antibody pretreatment and untreated groups(P > 0.05).4. The expression of TLR4, MyD88
and NF-κBp65 in colonic mucosa were significantly higher in all model groups than those in the corresponding control groups(P < 0.05, 0.01), RAD001 clinical trial and in groups of Tim-1 antibody pretreatment were significantly higher than those in untreated groups(P < 0.05). Conclusion: Tim-1 antibody treatment can aggravate mice colitis, decrease expression of Foxp3, and increase the expression of MyD88 and NF-κB p65, which suggest that Tim-1 antibody may aggravate the inflammation of IBD by down-regulating Foxp3 + Treg reaction and activating of TLRs/NF-κB signaling pathway. Key Word(s): 1. IBD;
2. Tim-1; 3. Treg cell; 4. Toll-like receptor; Presenting Author: TAO XU Corresponding Author: TAO XU Affiliations: the fourth hospital of jilin university Objective: To explore the diagnostic value of the anorectal manometry and gastrointestinal electroretinogram in constipated irritable bowel syndrome (IBS-C group). Methods: sixty patients with constipated irritable bowel syndrome and sixty healthy individuals (healthy control group) were recruited to take the anorectal manometry and gastrointestinal electroretinogram and were compared. Results: Anal AZD9668 sphincter maximum systolic blood pressure in IBS-C group were lower than those in healthy control group, which had a significant difference (P < 0.05). Residual pressure of the anal sphincter in IBS-C group were higher than those in healthy control group when simulate defecation, which had a significant difference (P < 0.01). Master
frequency, mean frequency and amplitude in IBS-C group is lower than those healthy control group particularly in the main frequency on an empty stomach in gastrointestinal electroretinogram. The frequency and amplitude in 3-mercaptopyruvate sulfurtransferase IBS-C group is lower than those healthy control group particularly in the amplitude in the postprandial. The area under the ROC curve were 0.625, 0.714, 0.883 respectively on the diagnosis of anorectal manometry, gastrointestinal electroretinogram and norectal manometry combined gastrointestinal electroretinogram. Conclusion: The sensitivity and specificity in the diagnosis of IBS-C with anorectal manometry joint gastrointestinal electroretinogram is higher than Anorectal manometry or gastrointestinal electroretinogram. It suggests that the combined detection has high clinical value. Key Word(s): 1. anorectal manometry; 2. gastrointestinal; 3. electroretinogram; 4.