8 and measuring absorbance at 260 nm. Acknowledgements We would like to thank Chia Y. Lee for kindly providing plasmid pMJ8426. TAP plasmid pSB1479 was selleck products obtained from Euroscarf http://web.uni-frankfurt.de/fb15/mikro/euroscarf/ord_tpla.html. This work was supported by the Biotechnology and Biological Sciences Research Council (United Kingdom). References 1. Shopsin B, Mathema B, Martinez J, Ha E, Campo ML, Fierman A, Krasinski K, Kornblum J, Alcabes P, Waddington M, et al.: Prevalence of methicillin-resistant and methicillin-susceptible Staphylococcus aureus in the community. J Infect Dis 2000, 182:359–362.CrossRefPubMed 2. National Nosocomial Infections Surveillance (NNIS) System Report,
data summary from January 1992 through June 2004, issued October 2004 Am J Infect Control 2004, 32:470–485. 3. Tiemersma EW, Bronzwaer SL, Lyytikainen O, Degener JE, Schrijnemakers AZD2281 P, Bruinsma N, Monen CHIR-99021 datasheet J, Witte W, Grundman H: Methicillin-resistant Staphylococcus
aureus in Europe, 1999–2002. Emerg Infect Dis 2004, 10:1627–1634.PubMed 4. Zetola N, Francis JS, Nuermberger EL, Bishai WR: Community-acquired meticillin-resistant Staphylococcus aureus : an emerging threat. Lancet Infect Dis 2005, 5:275–286.CrossRefPubMed 5. Hutchison CA, Peterson SN, Gill SR, Cline RT, White O, Fraser CM, Smith HO, Venter JC: Global transposon mutagenesis and a minimal Mycoplasma genome. Science 1999, 286:2165–2169.CrossRefPubMed 6. Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai K, Ashikaga S, Aymerich S, Bessieres P, et al.: Essential Bacillus subtilis genes. Proc Natl Acad Sci USA 2003, 100:4678–4683.CrossRefPubMed 7. Caldon CE, March PE: Function of the universally conserved bacterial GTPases. Curr Opin Microbiol 2003, 6:135–139.CrossRefPubMed 8. Comartin DJ, Brown ED: Non-ribosomal factors in ribosome subunit assembly are emerging targets for new antibacterial drugs. Curr Opin Pharmacol 2006, 6:453–458.CrossRefPubMed 9. Schaefer L, Uicker WC, Wicker-Planquart C, Foucher AE, Jault JM, Britton RA: Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus Methane monooxygenase subtilis. J Bacteriol 2006,
188:8252–8258.CrossRefPubMed 10. Wicker-Planquart C, Foucher AE, Louwagie M, Britton RA, Jault JM: Interactions of an essential Bacillus subtilis GTPase, YsxC, with ribosomes. J Bacteriol 2007, 190:681–690.CrossRefPubMed 11. Campbell TL, Daigle DM, Brown ED: Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function. Biochem J 2005, 389:843–852.CrossRefPubMed 12. Datta K, Skidmore JM, Pu K, Maddock JR: The Caulobacter crescentus GTPase CgtAC is required for progression through the cell cycle and for maintaining 50 S ribosomal subunit levels. Mol Microbiol 2004, 54:1379–1392.CrossRefPubMed 13. Matsuo Y, Morimoto T, Kuwano M, Loh PC, Oshima T, Ogasawara N: The GTP-binding protein YlqF participates in the late step of 50 S ribosomal subunit assembly in Bacillus subtilis.