12 0.12 ± 0.07B 0.19 ± 0.08A B 0.21 ± 0.15B 1.30 ± 1.85A PO4 3- (mg/l) 0.12 – 4.3 2.02 ± 1.40 0.33 ± 0.18A 4.81 ± 0.58A 2.16 ± 1.71A 3.98 ± 0.13A Values are means of triplicates ± Standard deviations (SD); Means with the same letter are not significantly different (P > 0.005). Summer (November to March); autumn (April to May); winter (June to August); spring (September to
October) TDS, Total p38 MAPK inhibitor dissolved solid; DO, Dissolved oxygen; COD, Chemical oxygen demand; NO3 -, Nitrate; NO2 -, Nitrite; PO4 3-, Orthophosphate. Antibiogram profile The susceptibilities of V. vulnificus (18 strains); V. parahaemolyticus (12 strains); V. fluvialis (19 strains) and V. metschnikovii (3 strains) to 21 different antibiotics by were examined. All the 52 isolates of Vibrio species were CUDC-907 clinical trial resistant to ampicillin and sulfamethoxazole, and sensitive to
imipenem, meropenem and norfloxacin. Vibrio fluvialis showed 100%, 90%, 70% and 80% resistances to trimethoprim, penicillin, cotrimoxazole and streptomycin, respectively, and 92%, 82% 90% and 100% of cephalothin resistances were exhibited by V. vulnificus, V. parahaemolyticus, V fluvialis and V. metschnikovii respectively. The results reveal the high individual and multiple antibiotics resistance among the test Vibrio strains (Additional file 1). Previous studies have shown that streptomycin, rifampicin, kanamycin, tetracycline, polymyxin B were active against Vibrio species [24], but this was at variance with our findings where we observed resistances SGC-CBP30 to streptomycin, tetracycline and polymyxin B in our Vibrio isolates. In this study, resistance to ampicillin was observed in all our Vibrio strains in difference to other studies that have been reported [25, 26], but corroborated by the findings of French and coworker [27] who reported similar antibiotics susceptibility profile for
V. parahaemolyticus. An increase in multi-antibiotics resistance bacteria in recent years is worrisome and the presence of resistance genes in bacteria Pregnenolone has further aided the transmission and spread of drug resistance among microbial pathogens [28]. Most studies on the antimicrobial susceptibility profiles of Vibrio species focus almost exclusively on clinical and/or food isolates with little information in the literature on those isolated from environmental sources such as treated municipal wastewater effluents. To our knowledge, this is the first study that specifically evaluated the antimicrobial susceptibility profile and detection of multiple antibiotics resistance genes of Vibrio strains isolated from treated municipal wastewater effluent in South Africa. The antibiotic resistance gene cluster and SXT element of Vibrio species strains In an attempt to finding a relationship between the multidrug-resistance phenotypes of V. vulnificus, V. metschnikovii, V. fluvialis and V.