2666), however this correlation was not as

2666), however this correlation was not as evident as Selleckchem VS-4718 the one estimated using the AFLP markers. FST values from the populations estimated using both techniques were compared. FST values of the five populations obtained for the VNTR analysis were lower than the FST values from the populations generated with the AFLP analysis, indicating that VNTRs detected a higher genetic flow between populations. Figure 4 Estimation of genetic populations of Xam in the Eastern Plains using AFLP and VNTR markers.

Xam isolates were assigned to the optimal number of clusters (K) estimated using STRUCTURE 2.3.3. A) Two genetic clusters estimated using AFLP data. B) Five genetic clusters estimated among isolates using VNTR data. Each isolate is represented by a single vertical line broken into K-colored segments. Color length in vertical lines represents the proportion of each inferred K clusters for each isolate. Color code of isolates labels represent the geographical origin of isolate: La Libertad: black; Granada: blue; Fuente de Oro: red and Orocué: green. Lines at the bottom delimit each estimated

genetic population (K). Fixation index (FST) is indicated for each population. The diversity of Xamhaplotypes in the Eastern Plains was comparable when the two types of molecular markers were implemented An analysis of haplotype assignment was CA4P manufacturer conducted to determine the number and distribution of haplotypes among sampled locations. A haplotype was defined with a 100% similarity threshold for both AFLP and VNTR loci. Both approaches generated a highly similar number of haplotypes for each sampled location and for reference strains (Table  3). In addition, both techniques allowed the distinction of a high number of haplotypes, with CYTH4 AFLPs and VNTRs detecting 86 and 87 haplotypes

out of 111 isolates, respectively. Consequently, the clonal diversity at each location was considerably high and comparable for both approaches (Table  3). However, high diversity values were most probably the result of the stringency in the assignment of haplotypes (100% similarity between isolates). Table 3 Assignment of haplotypes and clonal diversity in the Colombian Eastern Plains Molecular marker Location No. isolates No. haplotypes No. repeted haplotypes Corrected Nei’s index Corrected Shannon’s index Div_obs Div_obs AFLP La Apoptosis antagonist Libertad 47 33 4 0.967* 1.802* Granada 3 3 – 1.000 nan Fuente de Oro 1 1 – nan nan Orocué 50 39 7 1.000 nan Reference 10 10 – 0.985 2.001* Overall 111 86 13 0.991* 2.331* VNTR La Libertad 47 39 6 0.988* 2.163* Granada 3 3 – 1.000 nan Fuente de Oro 1 1 – nan nan Orocué 50 34 6 0.940* 1.783* Reference 10 10 – 0.978 1.653* Overall 111 87 12 0.984* 2.356* *Statistically significance (p > 0.05). nan: non calculated value because all isolates present a different haplotype. Haplotypes were divided in a minimum spanning network to visualize the connectivity between them (Figure  5).

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