5). Taken together, the data presented here demonstrate that the presence of already primed PVM-specific CD8+ T-cells at the time point of PVM-infection leads to enhanced control of viral loads and prevents T-cell-driven immunopathology. In conclusion, we have shown PVM-specific CD8+ T-cells provide partial protection
against PVM-induced disease, probably by preventing Th2 skewing of PVM-specific immune responses and by early control of viral loads. Our findings strongly suggest that pneumovirus vaccines designed to induce antigen-specific CD8+ T-cell memory may offer effective protection against pneumovirus-induced disease. Funding. This work was supported by Top Institute Pharma (T4-214); and the Wellcome Trust (WT 085733MA). CAL-101 purchase “
“Hepatitis A is an endemic illness in Brazil and mainly affects individuals during early childhood. However, because of improvements in sanitary conditions, the epidemiologic pattern of the disease has changed, and there has been an increase in the number of clinically evident cases in adolescents and adults [1]. In countries with low or intermediate rates of the disease (USA and Argentina), a routine pediatric vaccination program is thought to be the best strategy to SB203580 control hepatitis A virus (HAV) infection because children play a critical role in
disease transmission [2] and [3]. The epidemiological pattern and economic factors of HAV should be considered when selecting individuals and/or age groups for vaccination to prevent hepatitis A outbreaks. One strategy for understanding the epidemiology of hepatitis A is investigating immunity status by detecting anti-HAV antibodies in age-specific groups [4]. Although these studies, which are based on anti-HAV prevalence, are conventionally performed using serum samples, blood
collection by venipuncture is invasive and potentially painful [5]. Furthermore, the subsequent Rutecarpine transport (to avoid hemolysis), storage (temperature control), and processing (centrifugation) of serum samples require specific conditions that are mostly unavailable in surveillance settings. Thus, alternatives to blood analysis are needed that are non-invasive and easy to collect. Oral fluid could be a satisfactory and convenient alternative to blood analysis [6], particularly when considering children or other individuals from whom it is difficult to collect blood specimens as well as communities in difficult-to-access areas [7]. Although several studies have demonstrated the suitability of oral fluid as an alternative to serum for detecting HAV-specific antibodies [7], [8], [9] and [10], inadequate sensitivity and/or specificity of the available tests makes these assays inappropriate for clinical use. These features are intrinsically related to the pathogenesis of HAV infection and are critical for evaluating the antibody response that is induced by vaccination.