CCY9201 grown for 8 days under medium light in nutrient replete conditions. The average for all cyanobacteria cultures was
93.8 ± 2.9%. This translates to the dampening of a theoretical [F v/F m]Chla of 0.65 to [F v/F m]obs = 0.61 ± 0.02. We may expect that any combination of low [F v/F m]Chla , strong PBS fluorescence, and low F v/F m of the PBS pigments leads to a larger deviation of [F v/F m]obs from [F v/F Epigenetics inhibitor m]Chla . The two latter effects are illustrated in Fig. 10, where the results of Eq. 2 for all cyanobacteria are plotted against the variable fluorescence of the Gaussian component representing allophycocyanin [(F v/F m)APC, Fig. 10a] and the intensity of F 0 by allophycocyanin relative to Chla [(F 0)APC/(F 0)Chl, Fig. 10b]. The importance of [F v/F m]APC on the similarity between [F v/F m]Chla and [F v/F m]obs is clear, with the similarity expressed in Eq. 2 decreasing CRT0066101 mouse gradually as [F v/F m]APC <0.3. The results of Eq. 2 could not be explained by the allophycocyanin-to-Chla F 0 ratio plotted in Fig. 10b. This suggests that the variable fluorescence expressed by the PBS pigments is more important than the cellular pigment ratio in determining [F v/F m]obs. Fig. 10 Similarity of [F v/F m]obs and [F v/F m]Chla (Eq. 2) for cyanobacteria cultures expressed against
a variable fluorescence originating from allophycocyanin ([F v/F m]APC) and b against the ratio of allophycocyanin-to-Chla F 0. Fluorescence of the individual pigment components was assessed by Gaussian decomposition of F 0 and F m emission spectra with excitation at 590 nm Influence of detector
band width and spectral location on retrieval of F v/F m The signal-to-noise ratio of a fluorometer improves with increasing width of the emission slit. In addition, shifting the detection band to longer wavelengths reduces cross talk from the excitation source, which becomes important when excitation includes longer Phosphatidylethanolamine N-methyltransferase wavelengths (e.g. to excite cyanobacterial pigments). The variable fluorescence from cyanobacteria is sharply peaked at the PSII Chla emission band, in contrast to algae (Figs. 5, 7c). The emission detection band width must therefore be sufficiently narrow to retain sensitivity to the optical feature. The effect of the emission bandwidth and spectral location on observed F v/F m is illustrated in Fig. 11. F v/F m(590,λem) and F v/F m(470,λem) of cyanobacteria and algae cultures, respectively, were normalized to their peak and plotted as a function of λem between 620 and 750 nm, and for emission band widths ranging 10–50 nm. These spectra are highly conserved between all algae (Fig. 11a), with standard deviation of normalized F v/F m spectra <10% for wavelengths >665 nm (at shorter PI3K inhibitor wavebands coupling of different accessory pigments to PSII introduces some variability). In cyanobacteria (Fig.