In some experiments, cell culture supernatants were analyzed using luminex protein array according to the manufacturer’s instructions (Millipore). The frequency of antigen-specific cytokine producers was determined following culture for 24 h in 96-well filtration plates (Millipore), with or without 50 μg/mL MOG35–55. Antibodies buy PD98059 from eBioscience were: anti-IL-17 (TC11–18H10), biotinylated anti-IL-17 (TC11–8H4), IFN-γ (AN18), and biotinylated
anti-IFN-γ (R4–6A2). Streptavidin–alkaline phosphatase (Southern Biotech) and an alkaline phosphatase substrate kit (Vector Laboratories) were used to identify trapped cytokine. Spots were counted using the CTL ImmunoSpot Analyzer (Cellular Technology) with ImmunoSpot
software, and the number of spots in the medium-only wells subtracted to generate the data shown. Statistical analyses were performed using GraphPad Prism statistical analysis software. Group differences were analyzed by unpaired, two-tailed Students t-test. p-values of 0.05 or less were considered significant. This research was supported by a grant from the NINDS, NIH to B.M.S. (R01 NS057670) phosphatase inhibitor library and by the National Multiple Sclerosis Society Grant FG 1985-A-1 (S. J. L.). The authors declare no financial or commercial conflict of interest. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. “
“Citation: Ghazeeri G, Abdullah L, Abbas O. Immunological differences in women compared Adenosine triphosphate with men: overview and contributing factors. Am J Reprod Immunol 2011; 66: 163–169 Gender differences in the innate and adaptive immune systems have long been observed in humans. These immunological differences in immune function manifest as diverse susceptibilities to different types of infections and varied risks of developing autoimmune
disorders and maybe even, cancers. Several factors contribute to the development of this immunological dimorphism including sex hormones, genetic makeup, environmental causes, and more recently microchimerism. Although the aim behind this sexual immune dimorphism is still unclear, it is tempting to believe that the higher risk of developing autoimmune diseases in women somehow serves the higher evolutionary goal of reproduction and creating new life. “
“Pulmonary fibrosis is defined by an overgrowth of fibroblasts and extracellular matrix deposition, and results in respiratory dysfunction that is often fatal. It is the end stage in many chronic inflammatory interstitial lung diseases (ILD) such as sarcoidosis and idiopathic pulmonary fibrosis (IPF).