In this study, we monitored the effect of various monochromatic lights and plant growth regulators (PGRs) combinations on morphogenic and biochemical variation in wild grown-leaf derived callus cultures of Artemisia absinthium L. Combination of alpha-naphthalene acetic acid (NAA 1.0 mg/l) and Thidiazuron (TDZ 2.0 mg/l) resulted in optimum callogenic frequency (90%) when kept under fluorescent light for 4 weeks (16/8 h). In contrast
to the control (white spectrum), red spectrum enhanced peroxidase activity, protease activity, total protein content and chlorophyll a/b ratio. Green spectrum was found to be more supportive for total phenolics, total flavonoids and antioxidant activity. Yellow light enhanced MDA content while white and green MK-2206 light improved total chlorophyll content and carotenoid content. A positive correlation among callogenic response,
selleck products antioxidant activities and set of antioxidative enzyme activities was also observed in the current report. This study will help in understanding the influence of light on production of commercially important secondary metabolites and their optimization in the in vitro cultures of A. absinthium L. (C) 2013 Elsevier B.V. All rights reserved.”
“Studies on body composition and its determinants among SLE patients are limited. Estimation of body composition, analysis of determinants and associations of different body compartments are important in planning long-term care of these patients. The aim of the study was to identify the changes in body composition among SLE patients and assess the effect of corticosteroid use, patient and disease-related variables on body composition. We compared lean mass, fat mass, bone mineral
density (BMD), and bone mineral content (BMC) determined by dual-energy x-ray absorptiometry technology, in a group of premenopausal women with SLE (n=27) and an age-matched healthy group of women AZD1208 ic50 (n=27). The median (IQR) duration of SLE was 3 (2-5) years while median (IQR) duration and dose of prednisolone therapy were 108 (88-172) weeks and 9730 (6160-15360) mg, respectively. No significant difference was observed in body mass index (BMI) or total fat mass between the two groups. SLE patients, however, had significantly lower lean mass (p<0.001), BMD (p<0.001) and BMC (p<0.005) than healthy controls. Among cases, compared with lean mass, total body fat content showed stronger associations with total body BMD (r=0.49, p<0.01) and total body BMC (r=0.63, p<0.01). When a stepwise regression model was fitted, lean mass among controls and total fat mass among cases emerged as the best predictors of BMC/BMD. No significant correlations were found between the disease duration or cumulative glucocorticosteroid dose and total body BMD, total body BMC, lean mass or total fat content in SLE patients.”
“Mediastinal vascular malformations are interesting group of disorders, which are rarely seen in clinical practice.