One ribosome biogenesis factor in particular, KsgA, has been stud

One ribosome biogenesis factor in particular, KsgA, has been studied intensively for many years in E. coli. KsgA dimethylates each of two adenosines in the 3’-proximal helix (helix 44) of the small subunit rRNA [2] and serves as an important checkpoint in the assembly of the 30S subunit [3]. Cells lacking functional KsgA are often disadvantaged for growth when compared to wild-type cells. Specifically, knockout or mutation of ksgA in the organisms E. coli[3], B. subtilis[4], Mycobacterium tuberculosis[5], Yersinia pseudotuberculosis[6],

Chlamydia trachomatis[7] and Erwinia amylovora[8] is deleterious to cell growth, producing strains that either grow slower than or Selleck 10058-F4 are unable to compete efficiently with wild-type strains. In addition, knockout of ksgA in Y. pseudotuberculosis confers an PF-01367338 chemical structure attenuated virulence phenotype on the knockout strain [6]; inactivating mutations of ksgA in the plant pathogen E. amylovora decrease virulence [8]. A key observation to come out of the body of work on KsgA is that overexpression of catalytically inactive KsgA produces a dominant negative phenotype, being deleterious to both ribosome biogenesis and cell growth, thus suggesting KsgA might serve as https://www.selleckchem.com/products/Flavopiridol.html a potential antimicrobial drug target [3]. In

this context KsgA and its role in ribosome biogenesis and growth have been studied most extensively in E. coli. While ksgA gene knockouts have been tangentially studied in other organisms, no systematic study has been made of KsgA and its role in ribosome biogenesis and growth in another bacterial organism. In order to expand our knowledge of this system, we have extended studies of KsgA into the important Gram-positive human pathogen Staphylococcus aureus. Results Knockout of ksgA leads to a cold-sensitive phenotype To investigate the role KsgA plays in ribosome assembly and growth Ibrutinib cost we

generated an in-frame deletion of the ksgA gene in the S. aureus strain RN4220. The knockout strain was resistant to the antibiotic kasugamycin (Table  1); this resistant phenotype is also seen in E. coli. We confirmed the loss of KsgA activity in the cell by assaying purified 30S ribosomal subunits from both the wild-type (RN) and the knock-out (ΔksgA) strains for their ability to be methylated by exogenously added KsgA (Figure  1). As expected, subunits from the RN strain could not be further methylated by recombinant E. coli KsgA, while subunits from the ΔksgA strain could be efficiently methylated, albeit not to the same extent as E. coli 30S subunits. In addition to confirming the gene deletion, this experiment demonstrated that the structural requirements for KsgA binding to and methylating the small ribosomal subunit are conserved between E. coli and S. aureus. Table 1 Antibiotic resistance of RN4220 and Δ ksgA strains   MIC (μg/ml)   RN4220 ΔksgA Kasugamycin 800 >3200 Kanamycin 4 2 Paromomycin 4 2 Streptomycin 16 16 Figure 1 Activity assay.

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