SU11274 Tated analysis by linear traverse in circumferential

Third Line scans in the cortex were the egg before 180 meiotic spindle applied and advanced to the egg in a clockwise direction. SU11274 The Pixelintensit t Over an area about 5 m deep cortex top on the surface Averaged surface of the egg. In this analysis, the cortex adjacent to the spindle in the center of each meiotic t graph and is indicated by arrows. This took Ma Were not developed to the fluorescence intensity t Of the egg in the egg compare, but happier t show the Ver Changes in relative fluorescence t a region cortex alone Egg. As predicted from the images in Fig. 1, P Tyr fluorescence specific cortex appeared as a collection of variable intensity t Micro spikes at the periphery of the egg.
In unfertilized oocytes, the amplitude of the meiotic spindle N Hey micro spikes substantially Similar to those of the current in the rest of the cortex. But w During Tandutinib telophase anaphase was cortical fluorescence t 2 to 2.5 times h Contains ago in the hemisphere Lt native chromatin and the spindle. Localized concentrations of proteins Tyr P were consistently to the R Change of gel NDEs extrusion of Polk Observed body reflects m May receive the remains of the contractile ring. Pronuclear showed the fluorescence t in the cortex small signs of polarity T and was very variable with scattered areas of intense fluorescence distributed around the whole egg. Further observations at h Herer mag BEP evident than in the unfertilized egg P tires in the cortex, which is concentrated on the MII spindle.
Zus Tzlich Tyr P p in adjacent regions of the cytoplasm was concentrated Area of IBD. P Tyr was concentrated found at p Is the time in the majority of unfertilized eggs and completely Disappeared constantly after fertilization through anaphase. Another feature at the h Heren apparent magnification BEP was the observation of an intense P Tyr fluorescence in the plasma membrane or just above the cortex sperm, which recently merged with the egg. Semen to be the head in what seemed au Were outside of the plasma membrane is associated with a non-egg obtained FITTINGS cortical fluorescence. However, about 24 F Sleeve, in which the head of the sperm Just below the membrane of the egg plasma was positioned high P type tire labeling showed in the membrane.
The egg cortex and plasma The fact that this function only in a part of the eggs, the sperm were incorporated has been detected Noted that the Anh Ufung P Tyr may be a transient event. Examination of sperm Self-reported little or no time pTyr was detected in the head region, was the flagellum of spermatozoa Each of the Antique Bodies with P tires marked particularly pronounced in the area of gt The adapter. In summary, these results clearly show that the MII oocyte fertilization intensive PTK signaling, which is in the cortex, the To the spindle as well as specific points with p ‘S The time, the place of incorporation of associated meets sperm, and the website of the Polk Body extrusion. These pathways are volatile and are not detected in mid end pr