The calibration plots were linear over the range 0.1 to10 mu g/ml (r > 0.99). The limits of detection and quantification were 10 ng/ml and 50 ng/ml, respectively. The validation results demonstrated that the method is precise, accurate and selective for the determination of
Ko134 in mouse serum. The method was successfully applied to evaluate the pharmacokinetic parameters of Ko134 after different modes of administration in mice.”
“The aim of this study was to improve the texture of thermally processed carrots by selective pretreatments modifying plant-intrinsic properties. Pretreatments were a combination of a thermal or high-pressure (HP) treatment followed by a 1 h soak in a specific solution. Lowering the degree of methylesterification (DM) of the carrot pectin was confirmed to be one strategy to reduce texture click here degradation. The thermal or HP pretreatment resulted
in pectin with a lower DM which is less susceptible to beta-eliminative depolymerization. A subsequent Ca(2+) soak resulted in an even better texture by enhancing the amount of pectin cross-links within the cell wall. Lowering the pH of the carrots was proven to be another strategy. A thermal or HP pretreatment followed by soaking carrots in solutions of low pH proved to be effective in lowering the internal ACY-738 concentration carrot pH, hereby retarding beta-elimination and consequently texture degradation. The composition of the low pH solution was shown to be of importance; soak solutions
containing cations and/or Ca(2+) complexing agents have to be avoided. Ferulic acid proved to be a good acidifying candidate. In conclusion, for texture improvement of thermally processed carrots, lowering the susceptibility for beta-elimination and enhancing cell wall cross-links are two main targets which both can be reached by manipulating different plant-intrinsic selleck screening library properties. (C) 2010 Elsevier Ltd. All rights reserved.”
“To analyze the prognostic influence of patient characteristics, diagnostic markers or therapeutic procedures in women diagnosed with early ovarian cancer based on relapse and survival in long term follow-up.
All women diagnosed and treated for early ovarian cancer at our institution between 1992 and 2006 were included in this retrospective study. Patient characteristics, clinical data including operative procedure, serum markers, stage and histology at first diagnosis as well as follow-up data were analyzed with regard to survival times and relapse rates.
Altogether, 116 patients were included. Mean follow-up time was 7.0 +/- A 3.3 years (range 2-14 years). Histology revealed a serous tumor in 64.7% (75/116), mucinous in 19.0% (22/116) and endometiroid tumors in 7.8% (9/116) of all cases. TNM classification was pT1a in 49.1% (57/116), pT1b in 6% (7/116), pT1c in 32.8% (38/116) and pT2a in 12.1% (14/116). Lymph node involvement (N1) was found in 3.4% of all patients.