(2011). Fish, corals, and other invertebrates (Table 2) were collected from Bantayan Reef, Dumaguete (9° 19′ 56.1″ N, 123° 18′ 38.06″ E) across the SU-IEMS Marine Laboratory. Fish were collected by local fishermen using hand nets and fish traps. Experiments were conducted using four concrete tanks (3 m long × 1 m wide × 0.5 m deep) with
flow-through seawater at ambient conditions (mean temperature = 28 °C, salinity = 33 ppt, pH = 8.3). Half of each coral colony was Alectinib enclosed in a wire cage to ensure that a portion of every coral survived despite feeding activities of newly introduced A. planci ( Fig. 1). Coral fragments and colonies (∼15 cm L × W × H) were arranged in a way that the least preferred species were closest to the seawater inlet and the injected sea stars, while the most preferred species were farthest ( Pratchett, 2007). Fish and mobile invertebrates were also placed in the tanks. Eight sea stars GSK126 were separated in pairs and one A. planci was injected
with 10 ml oxgall (8 g l−1), oxgall (4 g l−1), peptone (20 g l−1), and TCBS (44 g l−1) at day 1 and the remaining one at day 4. All starfish were placed near the seawater inlet of Tanks 1–4, respectively. Interaction between all the animals in the tank was recorded for 4 h in the morning and 4 h in the afternoon using a GoPro Hero 2 HD video camera. Signs of disease such as darkened coloration to the skin and fins, erythema, changes to the eyes such as distension and cloudiness, periorbital swelling, haemorrhagic septicaemia and mortality were monitored every 8 h for 12 days. Mortality rates Cell press were highest in individuals injected
with bile derivatives (bile salts, oxgall) and TCBS, while mortality rates in peptones were moderate and only increased when concentrations were raised to 10–20× the standard concentration based on manufacturer formulation of TCBS (Fig. 2). Severity of clinical signs, mentioned hereafter, will range from low (i.e. localized to site of injection) to high (i.e. spread to more than 50% of the sea star). At the TCBS standard concentration of 10 g l−1, there was 0% mortality up to 48 h using Oxoid brand and only one 1 out of 10 A. planci died using Himedi brand. Most A. planci showed localized loss of turgor, matting, and mucus secretion. At half the TCBS standard concentration (5 g l−1), 50% of the sea stars showed loss of turgor and swelling after 8 h, but all recovered after 48 h and there was 0% mortality. At twice (20 g l−1) the TCBS standard concentration, 4 out of 10 exhibited localized tissue necrosis and 2 out of 10 sea stars showed medium severity necrosis at 8 h. After 24 h, 6 out of 10 showed medium severity necrosis and 1 out of 10 with localized necrosis.