10 Histologically, both are composed of nests of basaloid cells within the dermis. Although these differences are distinguishable in the majority of cases, there are cases in which distinction is difficult, not least in small and superficial biopsy specimens.9 The aim of this study was to compare the expression patterns of CD10 between BCC and
SCC and between BCC and TE. Additionally, the usefulness of this marker in the differentiation between these tumors was assessed and CD10 expression was evaluated in different histological subtypes of BCC. Materials and Methods Fifty-five cases of BCC, 50 cases of SCC, and 20 cases of benign adnexal tumor with follicular differentiation, #selleck kinase inhibitor keyword# including 13 cases of trichoepithelioma and 7 other Inhibitors,research,lifescience,medical benign adnexal tumors with follicular differentiation comprising trichoblastoma, trichoadenoma, sebaceoma, pilomatricoma, and pilar tumor were retrieved from the archives of the pathology departments of hospitals affiliated with Shiraz University of Medical Sciences. The specimens consisted of punch biopsy with adequate tumor tissue and excisional resection. Very tiny punch biopsies and poorly fixed specimens were excluded. H&E sections were reviewed by a dermatopathologist and were determined to be diagnostic cases of SCC, BCC, or other adnexal tumors. We classified 55 BCCs into 5 groups of superficial (1 case), nodular
(macro and micro) (38 cases), Inhibitors,research,lifescience,medical sclerosing/morpheic (3 cases), keratotic (4 cases), and basosquamous Inhibitors,research,lifescience,medical (9 cases). Immunohistochemistry was performed for all the specimens (125 cases). However, in this study, trichoepithelioma
was compared with BCC, which comprised the largest group of adnexal tumors of a follicular origin. This tumor has many overlapping histological features with BCC. Immunohistochemical staining was done on 5-µm sections obtained from formalin-fixed, paraffin-embedded blocks using the avidin-biotin peroxidase complex Inhibitors,research,lifescience,medical method. The primary antibody was mouse monoclonal antibody CD10 (Novocastra) (RTU-CD10-2), and the secondary antibody was Envision (K4061, Dako, Denmark). A judgment by the consensus of two independent observers was made as to the pattern of CD10 expression in all the cases. For each case, 10 fields were examined at high magnification (×400). Localization Chlormezanone of anti-CD10 to the stroma and/or tumor cells was determined in the cases with immunoreactivity as follows: negative (0-<10% positive cells); 1+, regionally positive (10-50% positive cells); and 2+, diffusely positive (>50% positive cells).8 Reactivity of the tumor cells was analyzed for central and/or peripheral staining. CD10 expression was compared with the positive control (perifollicular or peri-sebaceous gland area). Statistical Analysis The data were collected, tabulated, and statistically analyzed, using Statistical Package for the Social Sciences (SPSS).