Ectopic expression of HSP70 prevents receptor-dependent phosphorylation and nuclear translocation of Smad2, and blocks TGF-beta-induced epithelial-mesenchymal transition (EMT) in HaCat cells. Our findings reveal an essential role of HSP70 in TGF-beta-induced epithelial-mesenchymal transition (EMT) by impeding Smad2 phosphorylation.”
“Triclosan
was used as antibacterial agent in various vinyl thermoplastics and calcium carbonate (CaCO3)/thermoplastic composites and the antibacterial performances were studied through Halo and Plate-Count-Agar (PCA) test methods. The thermoplastics used were polyethylene (LDPE, MDPE, selleck inhibitor HDPE), polypropylene (PP), polystyrene (PS) and poly(vinyl chloride) (PVC). Escherichia coli (E. coli, ATCC 25922) and Stapphylococcus aureus (S. aureus, ATCC 25923) were used as the testing bacteria. The color index results suggested that introducing triclosan did not change the color of all thermoplastics used. The antibacterial results showed that the inhibition zone increased with increasing triclosan for nonpolar thermoplastics like LDPE, MDPE, HDPE, PP, and PS films whereas the opposite effect was observed for polar PVC film. The antibacterial efficacies of the triclosan decreased in the order of LDPE > MDPE > HDPE > PP > PS > PVC and this was confirmed by the triclosan releasing and FT-IR results. The differences in the antibacterial performances of the
studied thermoplastics with triclosan were associated with their rigidities, abilities to crystallize, Torin 2 cell line and free volume or molecular density. The sensitivities of E. coli and S. aureus bacteria to the triclosan were found to be dependent on the testing methods used for the antibacterial performance evaluations. The addition of CaCO3 worsened the antibacterial performances in the triclosan filled HDPE and PS blends, but had a benefit for improved bacterial reduction in the triclosan-filled IPI-145 clinical trial PVC blend. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 121: 253-261, 2011″
“Objectives. Although Emdogain is widely used as a gel in periodontal therapy, the exact mechanisms underlying
its regenerative ability still need to be further investigated. Therefore, we tested in vitro the effect of the product Emdogain on proliferation, viability, and migration of various human cell types of periodontium.
Study design. Proliferation and viability of alveolar osteoblasts (AOBs), epithelial cell line HSC-2, and human umbilical vein endothelial cells (HUVECs) were measured using [(3)H]-thymidine uptake and 3,4,5-dimethylthiazol-2-yl- 2,5-diphenyl tetrazolium bromide (MTT)-assay, respectively. Cell migration was investigated in microchemotaxis chamber.
Results. The proliferation and viability of AOB, HSC-2, and HUVECs were significantly stimulated by Emdogain (12.5-250 mu g/mL) in direct relationship with the amount of product present in the cell culture medium.